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[DOI] [PubMed] [Google Scholar] 150.Musial C., Kuban-Jankowska A., Gorska-Ponikowska M

Protein sequencing and subsequent cDNA sequence analysis provided evidence for the presence of Sec in the GPx-1 protein and proof that it was encoded by a UGA (opal) stop codon in the gene transcript

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After three rinses in PBS (10 min each), the tissues were incubated with secondary antibodies in PBS containing 5% BSA at room temperature for 2 h, washed three times with PBS, mounted using Mowiol mounting medium, and imaged under a fluorescence microscope

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Establish regular water breaks, if possible