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Forward primers for mutagenesis were: R38A: CTC CGC GAC TGC ATT CCA GGC GCT AAC CAA CAA GGT TTT TG R130A: CCC TTC CTC AGC CAA ACG CGG GCT TGG CAT TTT TTG Mutants were expressed and activity measured in membrane fractions as described 47

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doi: 10.1007/s00125-019-4959-1 9 VolpeCMOVillar-DelfinoPHDos AnjosPMFNogueira-MachadoJA

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Topical forms effectiveness varies with the formulation

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