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For all in vitro assays the NAPstar sensor protein was quantified by Bradford assay 77 and the sensor protein was used at a final concentration of 240480 nM

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Other left lungs were used for the harvesting of BALF, which was performed by lavaging the left lung three times with 500 l of saline via a tracheal catheter, while additional left lungs were weighed (wet weight)
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By contrast, when supernatant of PCA extract snap frozen in liquid nitrogen (F
Sleep improvements appear within the first one to two weeks
