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The next day, fixed tissues were washed three times in Dulbeccos phosphate buffered saline (DPBS, GIBCO, #14190-094), and depending on the purpose, either embedded, frozen in optimal cutting temperature (OCT) compound (Sakura, #4583) and stored at 20 C, or stored at 4 C in PBS containing 0.2% gelatin (Sigma Aldrich, #G1393), 0.5% Triton X-100 (Sigma Aldrich, #X100) and 0.01% Thimerosal (Sigma Aldrich, #T8784) (PBS-GT)

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The combination has been shown to produce greater effects on weight loss than either compound alone, making Cagrilintide a focus in combination studies involving GLP-1 analogs (Enebo et al.)

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