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First, the Henry nitroaldol reaction between nonanal and prenyl heptanoate 42 was performed with TMG, then trifluoroacetic anhydride (TFAA) and triethylamine were added in situ to eliminate the hydroxy group, and finally boron trifluoride diethyl etherate was added to the mixture to deprotect the acid and obtain 9( E )-9-NOME 32 with an efficient one-pot procedure (Scheme 71)

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Lack of oxygen permeation leads to necrosis of organoid centres, which adversely affects normal development and potential neuronal transfer pathways [84]

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