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Second, we fitted a cosinor mixed effects model using R package cosinoRmixedeffects, which includes random effects for mesor, amplitude and acrophase

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The lack of passaging capacity in the GF-HLCs is a limitation for certain applications but this mature phenotype makes the GF-HLCs ideal for in vitro studies of metabolism, biotransformation, and viral infection

This research was funded in part by Centro de Investigacin Biomdica en Red de Enfermedades Hepticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Subdireccin General de Investigacin Sanitaria, Ministerio de Economa y Competitividad (Madrid): EHD16PI02 (RF)