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The cells were cultured in Dulbeccos modified Eagle medium supplemented with 10% fetal bovine serum (Gibco), 100 g/mL streptomycin, and 100 U/mL penicillin in a humidified incubator at 37C in the presence of 5% CO 2

doi: 10.3389/fcimb.2017.00211 181 JarvisJ

A large number of causative genetic defects, which are localized to the nuclear genome or the mitochondrial genome, have been identified [9]
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