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The times were considered as the media of testing three times at a 1-hour interval

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The cDNAs were then added to the reaction with the indicated primers using SYBR Green Supermix (Bio-Rad Laboratories, Inc.) according to the manufacturer's instructions and amplified using qPCR, performed at 95C for 2 min, followed by 40 cycles of amplification at 95C for 10 sec, 62C for 30 sec and 72C for 30 sec, using a CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.)