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Data-dependent MS2 fragmentation for compound identification and annotation was performed via the AquireX workflow (Thermo Scientific), which comprised three deep scans with MS1 resolution at 60,000 and MS2 resolution at 15,000

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39,43,46,47,48 Dosages used have ranged from 800 to 2000 mg per day in longer-term studies (212 weeks), with the highest dose seeing the largest reductions in homocysteine