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While betaine and TMAO reductase are responsible for converting betaine and TMAO to TMA, respectively (Zuo et al., 2020)

[Google Scholar] 19.Frye RE, Melnyk S, Macfabe DF

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In this method, the extract was gradient eluted from a C18 column (Waters UPLC BEH C18-2.1 mm 100 mm, 1.7 m) using water and methanol, containing 0.05% perfluoropentanoic acid (PFPA) and 0.1% formic acid (FA)