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Some Rag2 / Il2rg / C57BL/6 J mice injected with purified TLR4 + ILC2s from HDM-sensitized WT C57BL/6 J mice also received 20 g of TLR4 monoclonal antibody 2 h before intranasal HDM challenge on day 1 to investigate the effect of blocking TLR4 on airway inflammation

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The sections were further stained with DAPI, washed with TBS and then mounted on slides with aqueous anti-fade mounting medium