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Cells were plated on top of the 0.6% Bacto agar layer in glucose and glutamine free DMEM media containing 10 mM glucose and 4 mM glutamine and supplemented with 10% FS and 0.3% Bacto Agar

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[DOI] [PMC free article] [PubMed] [Google Scholar] Dozio E, Ruscica M, Passafaro L, Dogliotti G, Steffani L, Pagani A, Demartini G, Esposti D, Fraschini F, Magni P