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After incubation for at least 1 h, the DTT was removed by loading the sample onto the bead bed of a MiniTrap desalting column pre-equilibrated with 100 mM HEPES p H 7.0

However, a huge amount of evidence demonstrated that multiple cell death modalities are synergistically involved in the pathogenesis of disease [201]

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Cells were cultured in EMEM (Eagle Minimum Essential Media, Sigma-Aldrich) supplemented with 10% FBS (Gibco), 200 mM L-glutamine (Sigma-Aldrich), 1% NEAA (Sigma-Aldrich) and maintained at 37 C, 5% CO 2