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Cells were fixed with 4% PFA in PBS at room temperature for 15 min, permeabilized with 1% saponin and stained by rabbit anti-ACP1 antibody (LifeSpan Bioscience) followed by Alexa 488-conjugated goat anti-rabbit IgG antibody (Invitrogen)

Miller HE, Bishop AJR

This cascade disrupts the electron transport chain, further increasing ROS generation and impairing ATP production, which is essential for sperm motility, as demonstrated earlier by Koppers et al [101]

& Tschopp, J

Mitochondrial uncoupling prodrug improves tissue sparing, cognitive outcome, and mitochondrial bioenergetics after traumatic brain injury in male mice