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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Protein refolding Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

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Covariate total MET was found to modify the association between vitamin B2 intake and osteoporosis (P interaction=0.0364), with the aforementioned relationship being more pronounced in the subgroup of insufficiently active individuals

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