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DNA methylation Genomic DNA was isolated from the liver tissue or cultured cells using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germantown, MD) following the manufacturers instructions
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However, at P10, both Dkk2 and Notum are undetectable regardless of the genotype (Supplementary Fig

Day 2 I had moderate nausea which made it the worst day
A consistent sublingual strip avoids that variability entirely every dose is the same as the last

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