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[DOI] [PubMed] [Google Scholar] 244.Ibrahim NA, Guo Z, Xu X

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Extracts were centrifuged at 20,000 g for 30 min, supernatant was filtered and separated on a 5 ml HisTrap (GE Healthcare) column in 50 mM Tris-HCl pH 8.0, 250 mM NaCl, 10 mM imidazole, with a linear gradient of imidazole (10500 mM)