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At 25 C, the conversion of 100 mM GSH was incomplete after 48 h and significantly poorer than that at 37 C, in which almost complete conversion was obtained (Fig

2 Chemistry of ROS The high reactivity of oxygen is attributed to the presence of two unpaired electrons in its outer orbital, which influence its chemical behavior and ability to form ROS

ER and Golgi stain were performed in Triton 0.1%, 20 min, RT

The oxidative stress parameters were assessed by measuring serum levels of total oxidant status (TOS) and total antioxidant capacity (TAC) by using a microplate spectrophotometer (Thermo Scientific Multiskan GO with SkanIt software 4.1) according to the protocol described by Hussain et al

Andrews NP, Prasad A, Quyyumi AA

doi: 10.1111/j.1574-6968.2007.00760.x
