gst glutathione affinity kd GST-Tagged Protein Purification: Comprehensive Guide & Uses Recombinant protein expression and purification:

The structural model essentially confirm previously proposed binding sites for glutathione, ATP and two Mg2+ ions, which appear identical for both catalytic steps

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However, an equimolar dose of dithiothreitol failed to increase GSH levels and protect mucosa from TNBS+ethanol-induced injury

Research Synergy: Why the Combination Is Studied