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The area under the curve was determined and normalized to the first timepoint

After 18 h, cells were fixed for 15 min with 3.7% paraformaldehyde and permeabilised for 5 min with 0.2% Triton X-100

These drawbacks have motivated the development of alternative strategies, including mesoporous silica carriers [77] and orally disintegrating tablets (ODTs) [92,126,127], to improve stability, prolong exposure, and achieve more flexible dosing