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This mixture was vortexed to mix properly and centrifuged at 13000 g for 30 min

Wang R, Zhang X, Ye H, Yang X, Zhao Y, Wu L, et al

Performed under the supervision of a trained medical professional

PMCID: PMC4825494

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According to the difference in the structure of binuclear Cu sites, oxidation state (Eoxy), deoxygenation state (Edeoxy) and reduction state (Emet) forms of catalytic sites have been generated in the process of melanin synthesis 3