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Before imaging, the growth medium was removed, then the cells were washed with PBS (2) and imaged in FluoroBrite DMEM

Free Ce6 and H-MnO 2 -PEG/C with or without H 2 O 2 added were incubated with SOSG (2.5 M), and then irradiated under 660-nm light with various periods of time in N 2 atmosphere (5 mW cm 2 )
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