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Redox-Active Small Molecule ArsR Sensors The remainder of the biochemically characterized ArsR family sensors are not metal ion or metalloid-sensing regulators, but instead use cysteine thiolate chemistry enabled by a precisely tuned p K a , nucleophilicity and topology to specifically sense either RSS or ROS (Figure 10E)

Prior to quantification, UMI sequences were corrected for sequencing errors, and valid barcodes were identified using the EmptyDrops method 87