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blood tests are prone to false positives and false negatives

see Figure 4

For the same reason, their long half-life allows them to subserve as second-messenger, signalling for important cellular responses in a mostly unknown fashion

Vitamin B12 and folic acid supplementation and plasma total homocysteine concentrations in pregnant Indian women with low B12 and high folate status

& Pardesi, K

One-way ANOVA was used to compare the mean values across multiple groups, followed by Dunnetts test for post hoc multiple comparisons to allowing for a detailed examination of significant differences between different experimental groups