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Duncan JD, Urbanowicz RA, Tarr AW, Ball JK

Hart WM

overhangs removed by Phusion DNA polymerase 120 ) carrying a sgRNA targeting KGA stop codon (5ATCTTGATGGATTGTTGTAA3) were co-transfected with a pUC19 with constructed donor sequence (1 kb 5 homology arm, gly-ser-thr linker, mKO2, gly-ser rich linker, 2 A self-cleaving peptide from porcine teschovirus-1, zeocin resistance, stop codon, and 1 kb 3homology arm) into HEK293 cells

doi: 10.1159/000422333

Then, there is the term proto-oncogene, a normal gene which, when altered by mutation, becomes an oncogene that can contribute to cancer

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