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For experiments involving intracellular staining of cytokines, T cells were stimulated for 3 h (mouse) and 6 h (human) with phorbol 12-myristate 13-acetate (PMA

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In fact, patients with CAP cannot avoid the risk of multimorbidity and polypharmacy, and some chronic diseases are even detected during the treatment of CAP

In the SSC, both WT and KI mice exhibited similar developmental patterns, with an increase in CCK-positive excitatory neurons and inhibitory interneurons postnatally, followed by stabilization and a gradual decrease in adulthood (Additional file 1: Fig

The change in barrier function (TEER) was then calculated using the time point specific reading (day 1, 4, 8 or 12) subtracted from the initial reference reading (day 0)

However, when deregulated, this pathway can be oncogenic in some tissues