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After slices were dehydrated in progressively stronger alcohols, cleaned in xylene, and mounted, hematoxylin was used as a counterstain ( Measurement of the percentage area of a positive SIRT -1 immunoreaction in hepatic tissue Images were taken using a digital camera (Toucan type BX53, Japan) linked to a computer and a light microscope (Olympus model BX53, Tokyo, Japan)

doi: 10.1038/nmeth.1923 19 LiHHandsakerBWysokerAFennellTRuanJHomerNet al

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These results show that H 2 O 2 -induced oxidative stress in cells leads to decreased viability, increased mtROS, reduced ATP levels, and downregulation of oxidative phosphorylation (OxPhos) genes, consistent with the changes induced by noise exposure in vivo

Due to their low antioxidant enzyme expression, -cells are especially vulnerable to oxidative damage