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qRT-PCR was performed in QuantStudio 3 realtime PCR system (Applied Biosystems, USA) using ChamQ SYBR qPCR Master Mix (Low ROX Premixed) (Q331-02, Vazyme)

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Endocrine 84:309319 Nappi A, Miro C, Cicatiello AG, et al (2025) The thyroid hormone activating enzyme, DIO2, is a potential pan-cancer biomarker and immunotherapy target
