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2.4 Analysis of index measurements 2.4.1 Liver histomorphometric analysis Liver tissues of 0.5 cm3 each were removed from Bournes fixative, dehydrated with different concentrations of alcohol using a biological tissue automatic dehydrator (KD-TS3A), paraffin-embedded tissues, trimmed the solidified wax blocks, and sliced with a tissue slicer (model: RM2235 slicer produced by leica, Graaf, Germany) with a thickness of 6 m