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Cells were cultured in DME media with 10 % fetal bovine serum and 1 % penicillinstreptomycin solution, in a humidified culture chamber at 37 C with 95 % air and 5 % CO 2

Finally, the tryptic peptides were desalted using SDB-RPS membrane (Sigma-Aldrich, 66886-U), vacuum-dried, and stored at 80 C before LC-MS/MS analysis

The administration of EGCG to the KYSE 510 esophageal cancer cell line, for durations spanning from 12 to 144 h, resulted in the hypermethylation of p16INK4a, RAR, MGMT and hMLH1 to be reversed in a manner that was dependent on time and concentration (29)

Br J Sports Med 51:636639

One possible explanation for such a response is that inhibition of mTORC1 and activation of GCN2 collectively suppresses global translation to conserve nutrients, while selective translation of mRNA such as ATF4 (mediated by increased p-eIF2) activates the expression of AARE genes to mitigate cellular stress

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