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Statements Author contributions ZL: Conceptualization, Validation, Writing original draft

We evaluated whether high CYS 2 caused any obvious bioenergetic effects, but did not observe xCT-dependent changes to mitochondrial oxygen consumption or extracellular acidification (Extended Data Fig

These findings collectively demonstrate that EC metabolism involves a coordinated sequence of intestinal absorption, hepatic and colonic biotransformation, and renal elimination, with distinct phase II conjugates acting as clearance intermediates and serving as characteristic metabolic signatures of EC exposure (Figure 3)