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For any in-vivo animal research conducted in the United Kingdom, full compliance with the Animals (Scientific Procedures) Act 1986 (ASPA) is mandatory, requiring valid Home Office licences and institutional ethical approval

Apatinib tyrosine kinase inhibitors entered clinical trials to test GC at the beginning, and it showed good efficacy in patients with GC after they failed chemotherapy

Controlled ovarian stimulation medicines (e.g., clomiphene, letrozole, gonadotropins) take rapid effect to initiate ovulation and need clinical supervision and monitoring

48 It exerts neuroprotective and neurotrophic effects, including BDNF upregulation and synaptogenesis

The DIA method included a survey scan (380980 m/z, resolution 240,000, AGC 500%, 5 ms injection) and MS/MS scans (1502000 m/z, 2 m/z isolation window, AGC 500%, 3 ms injection, normalized collision energy 25, cycle time 0.6 s)