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Microbial imbalances, marked by a reduction in beneficial genera such as Bifidobacterium , Akkermansia , and Roseburia , coupled with an increase in pro-inflammatory species like Bacteroides and Desulfovibrio , influence both endocrine signaling and immune function

Acta Endocrinol

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In vitro and animal studies suggest DIM has anti-inflammatory 17 and chemoprotective effects

The experimental design included 35 juvenile male Western albino rats, distributed into five groups of seven animals each: untreated controls, PPA alone, and PPA followed by treatment with omega-3, vitamin B12, or their combination

Histological analysis Synovial tissues were fixed in 4% paraformaldehyde, paraffin-embedded, and then transversely sectioned for routine HE staining