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Firefly and Renilla luciferase activities were measured according to the manufacturers guidelines (Beyotime, RG088S)

The development of several PAMs that are highly selective for mGlu 2 and have properties suitable for in vivo testing has enabled the identification of mGlu 2 as the major group II mGlu receptor subtype that could be pursued as a therapeutic strategy for reducing drug taking and seeking across a variety of classes of drugs

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IGFBPs, mainly IGFBP1 and IGFBP3, regulate IGF-1 activity via releasing and sequestrating IGF-1) [33, 34]