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Relation between the angiotensin-converting enzyme insertion/deletion polymorphism and blood pressure in Japanese male subjects

The final reaction comprises of 2 ml of Na-phosphate buffer (pH 7.3, 0.5 M,) 0.1 ml of enzyme extract, 0.1 ml of EDTA (3 mM) and 0.1 ml of H 2 O 2 (3 mM) for 5 min

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Churg A, Marshall CV, Sin DD, et al

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Glutamine is the most abundant amino acid and a crucial energy substrate, as well as an important nitrogen and carbon donor for various biosynthetic precursors