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CGA and its hydrolysates can suppress pathogenetic progression toward liver cancer via inhibition of MMP-9, a crucial factor involving the development of human HCC induced by HBV [324,325]

Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

Caution Use only on clean, dry skin

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Actual dosing is based on individual physician recommendations

doi:10.1016/S0895-4356(00)00340-1 160