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Proteins contain abundant sulfhydryl residues derived from their cysteine side-chains, which comprises up to 3% of the total amino acids in human [17]

2) Screening for Specific Diseases The ESR test could help with the diagnosis of certain diseases: Polymyalgia rheumatica (an inflammatory disease which causes muscle pain and stiffness) [27, 28, 29] Giant cell arteritis (inflammation of blood vessels) [30, 31, 32, 33, 34] Cancer [35, 36] Bone infections [37, 38, 39] Subacute thyroiditis (inflammation of the thyroid) [40, 41, 42] Ulcerative colitis [43] 3) Progression of Specific Conditions The ESR test cannot diagnose diseases, but it can track the progress of specific conditions [44]: Heart disease [45, 46, 47] Cancer [48, 5, 49] Rheumatoid arthritis [50, 51, 52] Systemic lupus erythematosus (SLE) [53, 54] Sickle-cell disease [55, 56, 57] 4) Serious Conditions ESR levels higher than 100 mm/hr could suggest a serious disease, such as infection, heart disease, or cancer [58, 5, 3, 6]

2.5 Cell culture MHCC97H, Hep3B, and JM1 cell lines were obtained from the National Collection of Authenticated Cell Cultures, Chinese Academy of Sciences

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With inhibition of heme synthesis, the iron that is not being incorporated into hemoglobin may precipitate in red blood cells, forming siderocytes (red blood cells with foci or short streaks of faint blue granules in their cytoplasm)