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The sections were rinsed of the cytoprotectant solution and incubated with 3% H 2 O 2 for 20 min, washed five times with KPBS, and stained with rabbit polyclonal primary antibody against TH (1:3,000), GFAP (1:3,000), or Iba-1 (1:1,000) in KPBS containing 0.4% Triton X-100 at 4 C overnight

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100 mg vial + 5 mL BAC water = 20 mg/mL A 2 mg dose = 0.1 mL = 10 units

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