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Cells were then transferred to ice, washed twice with cold Dulbeccos phosphate-buffered saline, and lysed in 180 l SDS Loading Dye (Cell Signaling Technologies, Danvers, MA)
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The hypothalamic-pituitary-adrenal (HPA) axis is supposed to wind down at night, but chronic stress keeps it firing
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Antioxidants & Redox Signaling, 2006
