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Concentration of testosterone was measured using Rat Testosterone ELISA Kit obtained from CAUSABIO Company, Houston, TX 77,054, USA, and Catalogue No.: CSB-E05100r

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Melanotan is not listed on the Australian Register of Therapeutic Goods, and has not been assessed for quality, safety or efficacy by the Therapeutic Goods Administration (TGA)

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High levels of leptin, which are common in individuals with obesity due to leptin resistance, fail to properly signal satiety, leading to continued food intake and reduced energy expenditure [32]