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The isolated C2 domains from PLC 1 and PLC 2 were previously reported to bind G q (Wang et al., 1999a), but the interface between the C2 domain alone and G q only buries approximately 400 2

Free cholesterol and sterols and corresponding esters were analyzed by liquid chromatography-tandem mass spectrometry, atmospheric pressure chemical ionization mode (APCI), and quantified using cholesterol-26,26,26,4,4,4-d6 as internal standard