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Janelia Fluor HaloTag ligand 554 (JFX554, CS315101, Promega) was added to cells at a working concentration of 20 nM and cells were grown for an additional 20 min

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There are three different UPR signaling pathways in ERS response that are mediated by (1) RNA-dependent protein kinase-like ER kinase (PERK), (2) activated transcription factor 6 (ATF6), and (3) inositol-required enzyme 1 (IRE1) (69)

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