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Assessment and characterisation of post-COVID-19 manifestations

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Proposed Mechanisms Preventing GABA receptor downregulation Counteracting compensatory NMDA glutamate receptor sensitization Stabilizing benzodiazepine receptor configuration (preventing shift to inverse agonist state) Upregulating GABAergic neurotransmission through pathways distinct from benzodiazepine action itself This tolerance prevention has profound clinical implications

Western blot Total protein extracts from macrophages (1 10 6 cells) were prepared using RIPA lysis buffer containing protease and phosphatase inhibitors (Thermo Fisher Scientific)