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Briefly, 100 ng recombinant PKC was incubated with 40 M ATP, 40 mM MgCl2, 2 mM CaCl2, 60 g/mL phosphatidylserine (PS), 2 g/mL dioleoylglycerol (DG) in Tris-HCl 20 mM pH 7.4 at 37 C for 30 minutes

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Many conditions associated with CPP are part of the broader chronic overlapping pain conditions recognized by the National Institutes of Health, which include fibromyalgia, chronic fatigue syndrome, chronic migraine, chronic tension-type headache, temporomandibular joint disorder, chronic low back pain, irritable bowel syndrome, interstitial cystitis/bladder pain syndrome, vulvodynia, and endometriosis