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Symptoms of vitamin D toxicity are due to increased calcium levels in the blood and can include nausea, vomiting, muscle weakness, neurologic or psychiatric symptoms, pain, loss of appetite, dehydration, increased urination, excessive thirst, and kidney stones

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Introduced in the 1980s, organotypic slice cultures were traditionally cultured using the roller-tube method but have since been optimized to culture via the air-liquid interface method, which allows slices to simultaneously receive nutrients from the culture medium and humidified air, facilitating long-term viability (Ghwiler, 1981

If all goes smoothly and your stomach acids to free up the B12, it can then attach to a protein called intrinsic factor, which facilitates B12s absorption into the bloodstream

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