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CoQ 10 triggers mitochondrial biogenesis against oxidative stress To determine whether oxidative stress triggers alteration of the intracellular mitochondrial network in ONH astrocytes and whether CoQ 10 treatment inhibits this alteration in mitochondria against oxidative stress, the mitochondrial morphology of ONH astrocytes were assessed by MitoTracker Red (Invitrogen-Molecular Probes, Eugene, OR, USA) staining, a marker for mitochondria

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Allow the fluid to flow to remove air from the tubing before intraocular irrigation begins

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Cell viability was assessed using the Cell Counting Kit-8 (CCK8) assay, measuring metabolic activity as an indicator of cell health