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Apoptosis was quantified using the CytoFLEX flow cytometer (Beckman)

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doi: 10.1016/j.nut.2010.01.014

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For each 100 l of solvent A, 8.7 l of solvent B was then added and vortexed to neutralize the sample, and the mixture allowed to sit on dry ice for 20 min

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