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Additionally, immortalized cell lines derived from testicular tissues, while lacking the complexity of native tissue, enable controlled mechanistic investigations of EED toxicity pathways

Glutathione and Inflammation Cytokines, such as TNF-, IL-1, and IL-6, are produced by immune cells and play a significant role in driving inflammation and triggering oxidative stress

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10.1038/s41586-018-0211-2 Nature 108 KamerK

This explains the lack of tolerance and dependence liability observed in animal studies