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The use of chelation for diagnostic purposes, following dental amalgam removal or in asymptomatic patients with baseline urine or blood levels approximating population norms was deemed inappropriate in 2005 by staff of the Agency for Toxic Substances and Disease Registry [85]
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At indicated times after washout, CMFDA (500 nM) was added for 1 h and the cells were lysed and processed for in-gel fluorescence binding assay, to assess recovery of CMFDA binding to GSTO1
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