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doi: 10.3389/fnut.2022.790250

Enzymatic assay for recombinant human HNMT An enzyme solution (0.1 M TrisHCl buffer [pH 7.4] containing 10 nM rhHNMT [Novus Biologicals, Centennial, CO, USA] and 0.4 mg/mL BSA) and substrate solution (0.1 M TrisHCl buffer [pH 7.4] containing 10 M histamine and 10 M SAM with or without ERGO) were separately preincubated at 37 C for 5 min, and 25 L of each solution was mixed together

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For example, the addition of varying concentrations of citrate to the diet of Drosophila on a high-calorie regimen resulted in a significant extension of lifespan, with higher concentrations of citrate also improving spontaneous activity and reproductive capacity in Drosophila

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